Actually these calibration frames (darks, flats, bias) will not help you reduce noise or improve detail
Yes, and worse, calibration frames
add noise.
To reduce noise, a very efficient method is called dithering, which can be done manually if you don't control your mount with a computer. By doing so, you also reduce the number of dark frames that have to be taken, 15 for example become sufficient.
If you don't dither, the more dark frames, the better.
More global exposure time will also improve your signal/noise ratio.
When it comes to bias frames, they are easy to take. They remove the offset signal. Just select the shortest exposure time of your DSLR, and take a shot in the dark. Now you have a bias frame. Even if they are quick to take, taking hundreds of them will not really help you much with noise reduction, as the effect would be invisible (they are substracted from flat frames). But if you really want to limit noise addition coming from a bias frame, with DSLRs, you can make a bias frame totally free from noise by mesuring the average offset signal of your DSLR and making a synthetic bias.
On light frames, you can limit the read noise by finding the optimal ISO setting. It depends on several parameters, such as the type of target you are shooting and the light pollution level. Usually, on deep sky objects, a good compromise is when the read noise starts to stabilize (after decreasing) when the ISO sensitivity rises. See
here for read noise charts on common DSLRs.
Be careful with over processing too.