Rodolphe:
The Moonlite focuser consumes between 36.195mm and 49.276mm of back focus from the minimum to the maximum of its travel. The travel is 13.081mm. This is with the Celestron 0.7 Focal Reducer installed. So, with the focal reducer, my imaging train is configured as follows:
Moonlite focuser - 42.7355mm (middle of the travel range)
Extension tube 1/4" (comes with focuser) - 6.35mm
ZWO OAG - 16mm
ZWO Camera adapter ring - 11mm (the black ring that came with your ASI1600 camera. I moved it to the other side of the EFW to help minimize vignetting)
ZWO EFW - 20mm
ZWO ASI1600mm - 6.5mm
Total back focus - 102.5585mm
The additional 3 are taken up in the travel of the focuser.
For use without the focal reducer, Moonlite includes a spacer that is inserted into the focuser in place of the focal reducer. This spacer consumes an additional 32.04mm of back focus. So, without the focal reducer, my imaging train is:
Moonlite spacer - 32.04mm
Moonlite focuser - 42.7355mm (middle of the travel range)
Extension tube 1/4" (comes with focuser) - 6.35mm
ZWO OAG - 16mm
ZWO Camera adapter ring - 11mm
ZWO EFW - 20mm
ZWO ASI1600mm - 6.5mm
Total back focus - 134.6255mm
The 1.3mm is taken from the travel of the focuser
The Celestron documentation says the back focus should be 105mm with and 133.35mm without the focal reducer. When I first began imaging, I set the travel on the focuser to perfectly hit those numbers before doing my first focus of the night. I did this by calculating the length needed in the travel and, knowing the step size from the motor as 0.00016" = 0.0041mm, calculated the number of steps to bring me to the exact length and set the focuser to that position before the initial focus. In truth, now I'm lazy and I simply set the focuser to the middle of the travel for both. Honestly, I can't see any difference. I'm not sure that 1 or 2 mm is that impactful, but that is just my experience. I would encourage you to experiment with your imaging train.
I hope you find that helpful.
Tim.