Wei-Hao Wang:
First you need to understand your instrument well. Then measure the star FWHM of every image you took. The FWHM is determined by both your instrument (optical aberration, diffraction, and tracking error) and seeing. Taking out the instrumental part, you are left with seeing.
This is where discussions of seeing can be confusing. There is the potential seeing based on meteorological conditions that MB purports to show, and then there's the resolution we each can realize of that potential with our own equipment, but when seeing conditions are talked about, I'm never sure which is meant. Note that there are also other seeing factors neither meteorological, nor mechanical, like imaging a target that happens to be just above a warm house on a cold night.... Anyhow, as Wei-Hao Wang said, over time you establish what your own very best fwhm is, and then your current seeing is measured off that based on images taken. The challenge then is determining if gear is optimized (best focus, collimation, backfocus, etc.) or if there's anything you can do to close the gap between potential and realized, and more challenging when there isn't any accurate current seeing conditions report to reference.
Seeing can also change dramatically through the night, so if I get started and find it's not worth imaging (about 75% of the time during the winter), I'll leave everything set up and then get up periodically to check if it's improved.
Cheers,
Scott