I'm running into a problem when trying to calibrate my Ha frames in Pixinsight. I'm using an ASI178MM-Cool which I know has some serious amp glow at longer exposures but up until this point, darks have been able to remove it quite successfully. The current problem is that the amp glow from the lower left and lower right is not being removed but the other parts are. This problem is not happening with my Oiii frames and they are actually 300 sec vs my 240 sec for Ha. I've tried image calibration several times and always get the same result. At this point, I think I may need to try and retake all of my dark frames. The current darks were taken at the same exposure (240sec), gain (279), and temp (-15C). In the attached image, the upper left is an uncalibrated light frame, upper right is a calibrated light showing the amp glow still present in the lower corners, and the bottom image is my master dark. Any thoughts as to why it is not being removed? Oh, and during the calibration process, I leave Calibrate and Optimize unchecked in the darks section of the Image Calibration tool in PI.

