Björn Arnold:
@Freestar8n has given an explanation in a post in another thread:
https://www.astrobin.com/forum/post/62215/
Therefore, I would take them at the same settings. Just to be sure to have the same sensor conditions
Björn
The underlying question is a good one. Just to review, there are three important things that flats correct:
1) Vignetting, which includes both light partially blocked by internal components in the optical train as well as dust motes.
2) Cos^4 radiometric light fall off, which is due to how irradiance decreases with field angle in any optical system.
3) Fixed pattern noise, which is due to PRNU or "photo response non-uniform. This is simply small variations in responsivity between pixels and it's a property of the sensor itself.
When we calibrate an image, the basic idea is to first subtract the dark signal and then divide that result by the master flat. Of course nothing is ever as simple as it seems so we have to consider how darks and bias offsets figure into the operation. When we take the flat data that will make up the master flat file, the data will contain both dark current and the bias offset. Here's the problem. In order to correctly divide the flat data it must be first normalized to have values between the values of [0,1] and that means that we must FIRST remove the dark current+bias offset before doing the normalization. Here it's important to recognize that the normalization process is
exactly the same as adjusting the gain on the sensor, which means that the sensor gain doesn't matter when you take flats! What does matter is that you take any flat-dark and/or any bias data at the same camera gain and temperature. That's important so that you are correctly subtracting the correct amount of dark signal and/or bias offset before scaling the flat data. Obviously if you are using a camera with negligible bias offset and exposures short enough that dark current is also negligible, you may not need to worry very much about removing that stuff from the Flat Master.
As a matter of good standard operating practice, it's always a good idea to use all the same settings on your camera when you take calibration data; but, in the case of flats, you can get often get away with using a different gain settings if you have too. Again, that depends a little on your camera, the exposure time that you use, and whether you have or need flat-darks and bias.
John