Hey Jeremy,
Knowing your imaging conditions, I must agree with the comments above. Calibration frames are almost as important as good lights. In you conditions the signal to noise ratio is pretty bad. Calibration frames will help clean it up. They can be taken at any time, not necessarily while imaging your lights. With the weather we have been having this winter and spring, any clear night is precious time for imaging lights and cannot be afforded to be hated on calibration frames.
Darks can be taken any time as long as there are taken at or near the same temperature. Same with bias. These are important to remove noise from the camera. On any long exposure, especially with an uncooled camera, the noise will increase with temp., and the longer the exposure the more noise will be generated from heat from the camera itself. Subtracting the noise will dramatically improve your lights.
Flats will remove any defects in your imaging train. Dust doughnuts, vignetting, etc. It will also help with pulling out faint details such as the dark lanes in you Iris image. When I started processing I was doing darks and bias . but not flats and I was asking the same question about my images. I found that flat fielding was the answer. Of all the gizmos and gadgets I have bought with this hobby, my Alnitak Flat Man was probably the best money I spent.
You can get good results with sky flats using a white tee-shirt over the objective. These grey skies we have had this spring are perfect to shoot flats. There are some good videos on you tube using this technique. These can be done anytime. no need to match the temp on these. Also if you try not to remove you camera from the train, or rotate it, you can reuse these over and over.
The same holds true for you darks and bias. You can use them over and over as long as they are at the same temp or close to your lights.
Clear Skies–MAYBE SOMEDAY!!!

Bob