No matter how many lights, you should have 30-40 darks. However, if you also have at least 30 lights, then the darks will be the most effective. My experience is as long as the darks are well matched with the lights, you will have all trace of amp glow removed. If you are using flats the same rules apply. Flat darks must be matched with flats, and there should be 30-40 of each. People with expensive equipment tend to use fewer control subframes because expensive cameras have fewer problems, but everyone could benefit with 30-40.
While flat darks must be matched with flats, flat + flat dark sets do not need to be matched with light + dark sets except for the camera's ISO.
I find that for many of my scopes/lenses I don't need flats+flat darks, but darks always make a big positive difference.
I make a set of darks with my set of standard settings for each of my cameras once a year and use that set with any lights I make that year. I have found 32 subframes each for darks, flats, and flat darks to be a convenient number.
To help your choose a standard setting for ISO, use this camera testing
site to help. You want as much dynamic range as possible, but also you want the highest ISO to get the shortest exposures. That is where the dynamic range curve starts being non-linear; for most Sony cameras it is at ISO 100. However, depending on your tracking or guiding you may need higher ISO to get shorter exposure times; in that case choose your ISO based on your desired exposure time.