This looks like it could be a shutter problem, depending on your shutter configuration, though a right-left shutter opening would be more common. This defect here would be from and up-down shutter, not sure how a right-left shutter would give this type of pattern. Shutters should last a long time, but I have blown through one shutter on one of my cameras in the past.
I assume you don’t see anything on your flats, since your flats are not correcting it? Because it might have been a problem with some lines of pixels on your sensor. Are you dithering? Still, seems unlikely if it is not in the flats.
Are all the images in the stack well aligned, if you have even a few images that are displaced then the edges and beyond the edge of those displaced images end up as lines or bands in the image.
One thing of that would do this type of horizontal banding is interference in the USB cable or a bad cable connection. Make sure there are no power cables close by. If you have another cable give that a try. If it is the cable you should see it even in a flat if you use exactly the same cable setup, though it is difficult to see problems looking at a flat. Still it is odd it is in the same place, unless there is a fault in the camera electronics that is triggered by the image acquisition.
You don’t need to be imaging at night I think to troubleshoot. Put your camera in a room facing a plain surface without any detail or shadows and take your exposures. You can experiment with cables etc to try to troubleshoot.
Rick