I'm having trouble with stacking photos lately and not sure what changed. I'm seeing dust mote artifacts in stacked & calibrated images that shouldn't be there.
Below is an image straight out of DSS. No further editing, STF applied. You can see dust motes in the top left and an overall red/green gradient from the filter. It's more prominent after DBE.

Extra details:
What I've tried based on other forum suggestions (none of them worked):
There's several nights of data. About 1/3 of them have lights and flats with those dust motes. The other 2/3 don't, as I cleaned off the sensor. Posted the two different flats & lights below:
https://drive.google.com/drive/folders/1LJgDgo7KcYTBPHdGFOGUQuozpX2Ie5lM?usp=drive_link
Something you'll notice is the dust mote in the flats are very noticeable, but in the light frame, they are really difficult to spot. I'm thinking that may be why? Perhaps that flat is over-calibrating?
There's also a slight rotation between all the frames (no more than 1°), but I doubt that's the issue.
Last comment, I usually avoid stacking in PI because my computer doesn't have the memory, but tried in FBPP. It gave me an error on the debayering process with one image, but I looked at the raw and calibrated version of that image and couldn't find any issues there either. But I did do a stack early on with about 80 frames in FBPP and the image came out just fine. So it's almost like some of the later images are causing issues, but I have no idea how to tell or how to fix.
Below is an image straight out of DSS. No further editing, STF applied. You can see dust motes in the top left and an overall red/green gradient from the filter. It's more prominent after DBE.

Extra details:
- Camera: ZWO ASI2600MC
- Lights are 300s
- Total lights: 408 frames
- Flats taken with a light panel
- Flats ADU average around 32,000
- Flat batches mostly in the range of 400-900ms (one outlier batch is 3.8s)
- Use 40 flats, 30 darks, 30 bias
- Everything is grouped by date in the stacking software
What I've tried based on other forum suggestions (none of them worked):
- Using dark flats in place of bias.
- Resetting DSS settings (Load > restore default settings)
- Changing the alignment settings to bilinear instead of automatic
There's several nights of data. About 1/3 of them have lights and flats with those dust motes. The other 2/3 don't, as I cleaned off the sensor. Posted the two different flats & lights below:
https://drive.google.com/drive/folders/1LJgDgo7KcYTBPHdGFOGUQuozpX2Ie5lM?usp=drive_link
Something you'll notice is the dust mote in the flats are very noticeable, but in the light frame, they are really difficult to spot. I'm thinking that may be why? Perhaps that flat is over-calibrating?
There's also a slight rotation between all the frames (no more than 1°), but I doubt that's the issue.
Last comment, I usually avoid stacking in PI because my computer doesn't have the memory, but tried in FBPP. It gave me an error on the debayering process with one image, but I looked at the raw and calibrated version of that image and couldn't find any issues there either. But I did do a stack early on with about 80 frames in FBPP and the image came out just fine. So it's almost like some of the later images are causing issues, but I have no idea how to tell or how to fix.