andrea tasselli:
Begging your pardon, it does. I never ever dithered the way suggested by you (possibly because my mounts aren't that good) and have never had an issue during post-processing hot-pixels. Besides, whether the pixel line up or not isn't an issue if the rejection criteria is based on position and you have enough instances to create a robust statistics. Certainly the way you suggest is even better, no doubt about it, but is tedious is you take a lot frames.
Actually John Kroon is correct, dithering serves more purpose than just to remove hot pixels; more important is fixed-pattern camera noise/artifacts. If you're using your processing software correctly, hot pixels shouldn't even survive to the stacking phase, given cosmetic correction options which can occur beforehand.
Therefore, a randomized dither is "technically" required, in order to properly handle fixed pattern camera noise. I say technically, because plenty of setups don't have enough integration time, or have adequate drift, to function "well enough" this way. Most images don't have enough integration time to really show this fixed-pattern noise, and most cameras have noise low enough to prevent this problem.
However, on my setup, I've had fixed-pattern noise persist in my final stacks (DSLR, 10+ hours integration time), even
with computerized dithering after
every frame, if I don't dither by great enough amounts. Pixel rejection cannot function properly without adequately-sized, truly randomized dithering, mathematically speaking. Hot pixels will definitely be handled fine by any type of dithering, but fixed-pattern noise will not. In fact, I read a mathematical analysis that showed dithering by drift leads to even
worse fixed-pattern noise than not dithering at all! It's likely you don't have such severe fixed-pattern noise, which is fine. I'm not saying one absolutely must dither in this way, however it is objectively better, when possible.
To Luc Aubut, you can absolutely dither manually! Many setups don't require it, however, so I recommend comparing a stack from a dithered night, to one without, and see if it matters. If it doesn't, you could save some valuable effort and time!