If you look at the ASIAIR log file, it clearly shows the autofocus procedure takes place between the subs. The next sub won't happen until guiding settles also. Here's an example from one of my recent log files:
2023/11/17 21:52:08 Exposure 300.0s image 22#
2023/11/17 21:57:09 Exposure 300.0s image 23#
2023/11/17 22:02:11 Exposure 300.0s image 24#
2023/11/17 22:07:12 [Guide] Dither
2023/11/17 22:07:12 [Guide] Dither Settle
2023/11/17 22:07:50 [Guide] Settle Done
2023/11/17 22:07:50 Start Tracking
2023/11/17 22:07:50 [Guide] Stop Guiding
2023/11/17 22:07:50 [AutoFocus|Begin] Run AF 2 hours later, exposure 3.0s Bin1, temperature 11.2℃
2023/11/17 22:07:50 Find Focus Star
2023/11/17 22:07:58 Find Focus Star: detect and calculate star size 3.2 , EAF position 31811
2023/11/17 22:07:58 Find Focus Star: finding appropriate stars star size 3.2
2023/11/17 22:08:13 Find Focus Star: detect and calculate star size 9.5 , EAF position 31961
2023/11/17 22:08:13 Calculate V-Curve
2023/11/17 22:08:21 Calculate V-Curve: detect and calculate star size 9.2 , EAF position 31961
2023/11/17 22:08:30 Calculate V-Curve: detect and calculate star size 7.4 , EAF position 31931
2023/11/17 22:08:39 Calculate V-Curve: detect and calculate star size 5.1 , EAF position 31901
2023/11/17 22:08:48 Calculate V-Curve: detect and calculate star size 3.7 , EAF position 31871
2023/11/17 22:08:56 Calculate V-Curve: detect and calculate star size 3.4 , EAF position 31841
2023/11/17 22:09:05 Calculate V-Curve: detect and calculate star size 3.1 , EAF position 31811
2023/11/17 22:09:14 Calculate V-Curve: detect and calculate star size 4.0 , EAF position 31781
2023/11/17 22:09:23 Calculate V-Curve: detect and calculate star size 5.5 , EAF position 31751
2023/11/17 22:09:32 Calculate V-Curve: detect and calculate star size 7.4 , EAF position 31721
2023/11/17 22:09:40 Calculate V-Curve: detect and calculate star size 9.5 , EAF position 31691
2023/11/17 22:09:47 Find Focus Point
2023/11/17 22:09:55 Calculate Focus Point: detect and calculate star size 4.4 , EAF position 31887
2023/11/17 22:10:04 Calculate Focus Point: detect and calculate star size 3.8 , EAF position 31872
2023/11/17 22:10:13 Calculate Focus Point: detect and calculate star size 3.5 , EAF position 31857
2023/11/17 22:10:22 Calculate Focus Point: detect and calculate star size 3.3 , EAF position 31842
2023/11/17 22:10:30 Calculate Focus Point: detect and calculate star size 3.3 , EAF position 31827
2023/11/17 22:10:30 Auto focus succeeded, the focused position is 31827
2023/11/17 22:10:30 [AutoFocus|End] Auto focus succeeded
2023/11/17 22:10:34 [Guide] ReSelect Guide star
2023/11/17 22:10:35 [Guide] Start Guiding
2023/11/17 22:10:36 [Guide] Guide Settle
2023/11/17 22:10:42 [Guide] Settle Done
2023/11/17 22:10:42 Exposure 300.0s image 25#
2023/11/17 22:15:43 Exposure 300.0s image 26#
2023/11/17 22:20:45 Exposure 300.0s image 27#